Drug for preventing and/or treating brain tumor and application thereof

A drug for preventing and/or treating brain tumors, wherein the drug comprises at least one Plasmodium strain;

• wherein the brain tumors are primary brain tumors or metastatic brain tumors;
• wherein the Plasmodium strain is cryopreserved and resuscitated Plasmodium strain;
• wherein the cryopreserved and resuscitated comprises the following steps:
• (1) cryopreservation of Plasmodium;
• (1.1) centrifuging whole blood containing Plasmodium at 300×g for 5 minutes, and transferring plasma to another 50 mL centrifuge tube;
• (1.2) removing leukocyte layer, and adding 2 volumes of 1640 culture medium, mixing well and centrifuging at 300×g for 5 minutes;
• (1.3) dropping an equal volume of 28% glycerol cryopreservation solution slowly into the 50 mL centrifuge tube, shaking and mixing well while adding, and incubating at room temperature for 5 minutes;
• (1.4) packing a RBC-glycerol cryopreservation solution mixture separately in cryopreservation tubes, 1.0 mL per tube;
• (1.5) putting the cryopreservation tubes into a cryopreservation box and directly storing the cryopreservation box into liquid nitrogen;
• (1.6) performing quality control by adding a proper amount of the RBC-glycerol cryopreservation solution mixture before cryopreservation to a proper amount of RPMI 1640 culture medium, culturing in an incubator with CO2 at 37° C. for 72 hours, and observing the color of the culture medium to confirm absence of turbidity;
• (2) resuscitation of Plasmodium;
• (2.1) preparing a 37° C. water bath cauldron, 50 mL centrifuge tubes, 3.5% NaCl solution, sodium chloride injection, a centrifuge, printed labels, and forms;
• (2.2) checking labels of cryopreservation tubes;
• (2.3) taking a cryopreserved Plasmodium blood and bath in water at 37° C. for 1 to 3 minutes, mixing well quickly and thawing;
• (2.4) transferring the cryopreserved Plasmodium blood to a 15 mL centrifuge tube, adding an equal volume of 3.5% NaCl solution slowly along the tube wall, pipetting gently and mixing well, standing at room temperature for 5 minutes, and discarding a supernatant;
• (2.5) adding 5 volumes of 0.9% NaCl solution along the tube wall, pipetting gently and mixing well, centrifuging at 300×g for 5 minutes, and discarding a supernatant;
• (2.6) adding normal saline to achieve 50% hematocrit, mixing well, counting and recording red blood cells density, and storing temporarily at 4° C. for clinical inoculation;
• (2.7) performing quality control by smearing remaining blood samples, preparing blood smears, and observing parasitemia and Plasmodium status via microscopy before injection, and recording.