Biofumigant composition and a process for the preparation thereof

A process for preparing a biofumigant composition, wherein the process comprises:

• (a) drying aerial parts of A. vulgaris at a temperature in a range of 30-40° C. for a duration of 48 hours, and powdering the dried aerial parts to obtain a dried powder;
• (b) extracting the dried powder with an organic solvent to obtain an extract;
• (c) evaporating the extract under vacuum to obtain a residue, and dissolving the residue in 15-20 ml of methanol;
• (d) fractionating the methanolic extract obtained in step c) and subjecting it to silica gel column chromatography followed by eluting with a polar solvent to obtain an active insecticidal fraction;
• (e) pooling, and concentrating the insecticidal active fraction of step d) and further purifying by flash chromatography followed by preparative high performance liquid chromatography (HPLC) to obtain a purified fraction;
• (f) obtaining a bioactive compound in a range of 90-95% from the purified fraction;
• and adding lemon grass oil to obtain a biofumigant composition.