Embryogenesis factors for cellular reprogramming of a plant cell

A method of obtaining genome-modified, doubled haploid plant progeny, the method comprising:

• (a) obtaining a first plant transformed with one or more expression cassettes comprising:
• (i) a polynucleotide sequence encoding a morphogenic developmental polypeptide, wherein the morphogenic developmental polypeptide is a WUS/WOX homeobox polypeptide, a Babyboom (BBM) polypeptide or an Ovule Development Protein 2 (ODP2) polypeptide, or a LEC1 polypeptide;
• (ii) a polynucleotide sequence encoding an embryogenesis factor, wherein the embryogenesis factor is a maize cyclin delta-2 polypeptide comprising an amino acid sequence at least 95% identical to SEQ ID NO:
• 21;
• and
• (iii) a polynucleotide sequence encoding a Cas polypeptide, wherein the Cas polypeptide enables genomic modification of a DNA target site in the first plant, wherein at least one guide RNA is provided for the Cas polypeptide, the guide RNA comprising a region complementary to the DNA target site;
• wherein the Cas polypeptide and the guide RNA form a complex that binds and modifies the DNA target site resulting in a modified target site;
• (b) obtaining a second plant;
• (c) pollinating the first plant with pollen from the second plant;
• (d) obtaining embryogenic haploid progeny, wherein the haploid progeny comprises the genome of the first plant but not the second plant, and wherein the maize cyclin delta-2 polypeptide stimulates an embryogenic cell fate resulting in the embryogenic haploid progeny;
• (e) obtaining doubled haploid progeny from the haploid progeny, wherein the maize cyclin delta-2 polypeptide stimulates diploidization of the haploid progeny, resulting in the doubled haploid progeny;
• and
• (f) selecting genome-modified, doubled haploid plant progeny,
• wherein the maize cyclin delta-2 polypeptide increases the frequency of embryogenic cell fate and diploidization in the haploid progeny relative to a non-treated control.