Position adjusting method, microparticle analysis device, and program

A non-transitory computer readable storage medium containing program instructions for allowing a microparticle analysis device to execute:

• an imaging step of imaging, while moving a flow path through which a microparticle is able to flow in an optical axis direction, the flow path in a plurality of positions in the optical axis direction;
• a movement step of moving the flow path in the optical axis direction on a basis of a focus index for each of a plurality of images acquired at the imaging step;
• an illumination step of illuminating the flow path with a first laser light source at a wavelength range between 550 nm to 800 nm, a second laser light source at a wavelength range between 450 nm to 550 nm, and a third laser light source at a wavelength range between 380 nm to 450 nm
• using a plurality of mirrors to redirect a condensed beam of the first laser light source and the second laser light source and the third laser light source at angles into additional detectors and sensors;
• using a diffusion plate that diffuses the condensed beam of the first laser light source and the second laser light source and the third laser light source, the diffusion plate being located in between a mirror and an objective lens;
• and
• an adjustment step of specifying a feature position of the flow path from an image of the flow path in a position after movement at the movement step, and adjusting a positional relationship between the feature position and a reference position in a direction perpendicular to the optical axis direction.