Method for detecting nucleic acid
AssigneeInstitute of Zoology, Chinese Academy of Sciences
InventorsWei Li, Qi Zhou, Fei Teng
Life of this patent
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Abstract
A method for detecting the presence and/or amount of a target nucleic acid molecule in a biological sample and a related kit. The method comprises: (a) contacting the biological sample with: i) Cas12b protein, ii) a gRNA targeting a target sequence of the target nucleic acid molecule, and iii) a single-stranded DNA reporter molecule generating a detectable signal after being cleaved to form a reaction mixture; and (b) detecting the presence and/or level of the detectable signal generated in the reaction mixture.
Claims19
A method of detecting the presence and/or amount of a target nucleic acid molecule in a biological sample, the method comprising the following steps:
- (a) contacting the biological sample with:
- i) a Cas12b protein, ii) a gRNA directed against a target sequence in the target nucleic acid molecule, and iii) a single-strand DNA reporter molecule which produces a detectable signal after being cleaved, thereby forming a reaction mixture;
- (b) detecting the presence and/or level of the detectable signal produced in the reaction mixture,
- wherein the presence and/or level of the detectable signal indicates the presence and/or amount of the target nucleic acid molecule,
- wherein the Cas12b protein comprises an amino acid sequence having at least 95% sequence identity with SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.