Device and methods of using device for detection of aminoacidopathies
AssigneesUniversity of Maryland, College Park, Children's National Medical Center
InventorsOmar Bilal Ayyub, Brian Theodore Heligman, Peter Kofinas, Marshall Lynn Summar, Juan Manuel Cabrera-Luque, Gary Cunningham
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Abstract
The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.
Claims25
A biosensor comprising:
- a light source;
- a light detector comprising a complementary metal-oxide semiconductor (CMOS) or a charged-coupled device camera;
- and
- one or more reaction surfaces comprising at least one oxidizing agent lyophilized or desiccated onto the one or more reaction surfaces, and one or more metabolic enzymes or functional fragments thereof;
- wherein at least one of the one or more metabolic enzymes or functional fragments thereof is at least 80% homologous to a phenylalanine dehydrogenase from Geobacillus thermoglucosidasius;
- wherein the one or more reaction surfaces do not comprise and are not attached to an electrically conductive support;
- and
- wherein the one or more reaction surfaces are free of an electron mediator;
- and
- wherein the light source is positioned at a distance from at least one of the one or more reaction surfaces sufficient to irradiate the at least one of the one or more reaction surfaces and the light detector is positioned at a distance from the one or more reaction surfaces sufficient to collect irradiated light from the one or more reaction surfaces, and
- wherein the biosensor further comprises a housing that contains at least a first fluid opening adjacent to and in fluid communication with a filter paper immediately adjacent to a microfluidic chamber that comprises at least one of the one or more reaction surfaces.
Dependencies are inferred from the claim text.