Method to detect and enumerate microorganisms
AssigneeNeogen Food Sagety US Holdco Corporation
InventorEvan D. Brutinel
Life of this patent
Estimating the term…
Abstract
A method. The method includes providing a device, the device comprising: a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces; and a layer of adhesive-coated on the upper surface of the substrate, the adhesive being non-inhibitory to the growth of microorganisms; and a cold-water-soluble powder adhered to the adhesive, the powder comprising: guar gum; one or more nutrients for growing microorganisms; and a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; incubating a sample in the device to form a gel; and counting colonies between 24 hours to 72 hours and/or determining if the gel surrounding a colony is degraded.
Claims13
A method, comprising
- providing a device, the device comprising:
- a body member comprising a self-supporting, waterproof substrate having upper and lower surfaces;
- and
- a layer of adhesive coated on the upper surface of the substrate, the adhesive being non-inhibitory to growth of microorganisms;
- and a cold-water-soluble powder adhered to the adhesive, the powder comprising:
- guar gum;
- one or more nutrients for growing microorganisms;
- and
- a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member;
- incubating a sample in the device to form a gel;
- and
- counting colonies of said microorganisms said microorganism colonies between 24 hours to 72 hours and determining if the gel surrounding said colonies is degraded by an imaging system or by visual inspection and an imaging system;
- and
- wherein determining if the gel surrounding said microorganism colony is degraded comprises pressing the coversheet to determine if there is a movement of water.
Dependencies are inferred from the claim text.