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US 12,540,340 B2Granted
Processes of producing fermentation products
AssigneeNovozymes A/S
InventorsThomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
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Abstract
The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
Claims20
A process for producing ethanol from a starch-containing material comprising corn, comprising the steps of:
- i) liquefying the starch-containing material at a temperature between 70-95 degrees centigrade using an alpha-amylase;
- ii) saccharifying using a glucoamylase;
- iii) fermenting using a yeast fermenting microorganism to produce the ethanol;
- and
- wherein a cellulolytic composition comprising an endoglucanase, a beta-glucosidase, and a cellobiohydrolase is added during saccharification or fermentation, wherein ii) saccharifying and iii) fermenting are done simultaneously, and the beta-glucosidase has at least 90% sequence identity to amino acids 20 to 863 of SEQ ID NO:
- 6, comprises one or more substitutions selected from the group consisting of L89M, G91L, F100D, I140V, I186V, S283G, and N456E, and has a Relative ED50 loading value of less than 1, and wherein the ethanol yield is increased compared to the ethanol yield obtained without the cellulolytic composition and wherein sequence identity is determined using the Needleman-Wunsch algorithm as implemented in the Needle program of the EMBOSS package, version 5.0.0, using a gap open penalty of 10, a gap extension penalty of 0.5, and EBLOSUM62 substitution matrix.
3 more elements
Dependencies are inferred from the claim text.