Methods and compositions for reducing pod shatter in canola

A method for producing a canola plant or plant part thereof comprising at least one cell having a mutated endogenous SHATTERPROOF MADS-BOX(SHP) gene, the method comprising:

• contacting a target site in an endogenous SHP gene in the canola plant or plant part thereof with a guide nucleic acid and a nuclease comprising a cleavage domain and a nucleic acid binding domain, wherein the guide nucleic acid binds to the target site and the target site is in a region of the endogenous SHP gene having at least 95% sequence identity to any one of SEQ ID NOs:103, 112, 243, or 261, wherein the nucleic acid binding domain binds to the target site in the endogenous SHP gene, and wherein the endogenous SHP gene
• (a) comprises a nucleotide sequence having at least 95% sequence identity to any one of SEQ ID NOs:69, 70, 100, 101, 148, 149, 177, 178, 206, 207, 240 or 241;
• (b) comprises a region having at least 95% sequence identity to a nucleotide sequence of any one of SEQ ID NOs:72-96, -103-144, 151-173, 180-202, 209-236, 243-288 or -324-338;
• (c) encodes a SHP polypeptide having at least 95% sequence identity to any one of SEQ ID NOs:71, 102, 150, 179, 208, or 242;
• and/or
• (d) encodes a region of a SHP polypeptide having at least 95% sequence identity to any one of SEQ ID NOs:97-99, 145-147, 174-176, 203-205, 237-239 or 289-291, thereby producing the canola plant or plant part thereof comprising the at least one cell having a mutation in the endogenous SHP gene to provide the mutated endogenous SHP gene.