Peroxidase activity towards 10-acetyl-3,7-dihydroxyphenoxazine
AssigneeCodexis, Inc.
InventorsDa Duan, Auric Anthony Sowell-Kantz, Aksiniya Lyubenova Petkova, Jovana Nazor, Nandhitha Subramanian, Oscar Alvizo
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Abstract
The present invention provides engineered peroxidase enzymes, polypeptides having peroxidase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing peroxidase enzymes are also provided. The present invention further provides compositions comprising the peroxidase enzymes and methods of using the engineered peroxidase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.
Claims16
A polynucleotide sequence encoding at least one engineered peroxidase comprising a polypeptide sequence having 90% or more sequence identity to SEQ ID NO:
- 4 wherein said engineered peroxidase comprises a substitution in said polypeptide sequence at position 393, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 4, and wherein said engineered peroxidase comprises at least one improved property compared to wild-type Anabaena (Nostoc) sp. peroxidase.
A polynucleotide sequence encoding at least one engineered peroxidase, said polynucleotide sequence comprising 90% or more sequence identity to SEQ ID NOS:
- 3 wherein the polypeptide sequence of said engineered peroxidase comprises a substitution at position 393 and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 4 and wherein said engineered peroxidase comprises at least one improved property compared to wild-type Anabaena (Nostoc) sp. peroxidase, wherein said improved property comprises improved activity on a substrate comprising 10-acetyl-3,7-dihydroxyphenoxazine.
A polynucleotide sequence encoding at least one engineered peroxidase comprising a polypeptide sequence having 90% or more sequence identity to SEQ ID NO:
- 4, or a functional fragment thereof, wherein said engineered peroxidase comprises a substitution in said polypeptide sequence at position 393, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 4, and wherein said engineered peroxidase comprises at least one improved property compared to wild-type Anabaena (Nostoc) sp. peroxidase wherein said polynucleotide comprises an odd-numbered sequence of SEQ ID NOS:
- 5 and 171-289.
Dependencies are inferred from the claim text.