US 12,540,310 B2 · Differentiation method of neural stem cells manufactured by direct cell conversion into astrocytes

Abstract

The present invention relates to a method for efficiently differentiating neural stem cells into astrocytes and, more particularly, to a cell conversion-based method for more efficiently differentiating human neural stem cells into astrocytes that exhibit immune response suppression ability within a short period of time. Unlike a conventional method, the method for differentiating neural stem cells into astrocytes by using a differentiation medium containing a combination of several cytokines, according to the present invention, involves a shortened differentiation time and has excellent differentiation efficiency, and the differentiated astrocytes exhibit immune response suppression ability, and thus can be useful as an agent for treating various brain diseases such as degenerative neurological diseases.

Claims14

Dependent claims

A method for differentiating neural stem cells into astrocytes comprising:

(a) primarily culturing neural stem cells for only 2 days in a differentiation medium containing ciliary neurotrophic factor (CNTF), basic fibroblast growth factor (bFGF), and bone morphogenetic protein 4 (BMP4);
(b) secondarily culturing the primarily cultured cells for only 2 days in a differentiation medium not containing bFGF, but containing CNTF and BMP4;
and
(c) tertiarily culturing the secondarily cultured cells in a differentiation medium not containing CNTF, bFGF and BMP4, but containing fetal bovine serum (FBS);
thereby producing astrocytes.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/425,189Filed 20 Jan 2020

Priority

KR 10-2019-000825622 Jan 2019

Published

US 2022/0119765 A121 Apr 2022

Granted

3 Feb 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 5/0622
Cells of the nervous system: glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
A61K 35/30
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61P 29/00
Non-central analgesic, antipyretic or antiinflammatory agents
C12N 2501/11
Growth factors: epidermal growth factor [EGF]

Differentiation method of neural stem cells manufactured by direct cell conversion into astrocytes

Life of this patent

Differentiation method of neural stem cells manufactured by direct cell conversion into astrocytes

Life of this patent

Abstract

Claims14