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US 12,539,515 B2Granted
Devices for cell separation
AssigneeSouthwest Research Institute
InventorsJian Ling, Stephen T. Wellinghoff, Carlos Martín Cantú, Angelica Fernandez
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Abstract
Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and/or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.
Claims10
A method to separate one or more targeted cells from a plurality of cells comprising:
- (a) supplying a device comprising biocompatible polymer material having a plurality of voids having a diameter D and a plurality of pore openings between said voids having a diameter d, including a surface area for cell separation, wherein 90% or more of said voids have a selected void volume (V) that does not vary by more than +/−10.0% and 90% or more of said pore openings between said voids have a value of d that does not vary by more than +/−10.0%;
- (b) providing a surface coating on said voids wherein said surface coating provides for cell binding;
- (c) passing a plurality of cells in a fluid through said device to provide a fluid output;
- and
- (1) capturing one or more selected cells from said plurality of cells on the coated surface of said device;
- or
- (2) capturing the one or more selected cells from the plurality of cells in said fluid output.
4 more elements
Dependencies are inferred from the claim text.