Norovirus S particle based vaccines and methods of making and using same
AssigneeChildren's Hospital Medical Center
InventorsMing Tan, Xi Jiang
Life of this patent
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Abstract
Disclosed herein are vaccine compositions, in particular, polyvalent icosahedral compositions for antigen presentation. The disclosed compositions may contain an S particle made up of recombinant fusion proteins. The recombinant fusion proteins may include a norovirus (NoV) S domain protein, a linker protein domain operatively connected to the norovirus S domain protein, and an antigen protein domain operatively connected to said linker.
Claims20
A method of immunizing an individual against a rotavirus infection, comprising administering to the individual a vaccine composition comprising
- a) a norovirus (NoV) S domain protein comprising, with reference to SEQ ID NO:
- 25, an alanine at position 69 and a cysteine at positions 57, 58, and 136;
- b) a linker protein domain operatively connected to the C terminus of the norovirus S domain protein;
- and
- c) a human rotavirus spike protein VP8 antigen operatively connected to the linker protein domain.
A vaccine composition platform comprising a sequence selected from SR69A (SEQ ID NO:
- 27), SR69A/V57C/M140C-VP8 (SEQ ID NO:
- 31), SR69A/V57C/Q58C/S136C-VP8 (SEQ ID NO:
- 33), or SR69A/V57C/Q58C/S136C-VP8 (SEQ ID NO:
- 33).
A bacterial expression system comprising E. coli expressing a sequence selected from SR69A (SEQ ID NO:
- 27), SR69A/V57C/M140C-VP8 (SEQ ID NO:
- 31), SR69A/V57C/Q58C/S136C-VP8 (SEQ ID NO:
- 33), or SR69A/V57C/Q58C/S136C-VP8 (SEQ ID NO:
- 33).
Dependencies are inferred from the claim text.