Super-resolution microvessel imaging using separated subsets of ultrasound data

A method for generating an image of microvasculature in a subject from ultrasound data, the method comprising:

• (a) accessing ultrasound data acquired from a subject with a computer system;
• (b) generating microbubble signal data by extracting microbubble signals from the ultrasound data using the computer system;
• (c) generating a plurality of subsets of microbubble signal data from the microbubble signal data using the computer system, wherein the plurality of subsets of microbubble signal data are generated by separating the microbubble signal data into subsets of microbubble signals based on acoustic properties of individual microbubbles, wherein the acoustic properties of individual microbubbles comprise at least one of intensities of the microbubbles or blinking behaviors of the microbubbles;
• (d) generating a microvessel image for each subset of microbubble signal data using the computer system, wherein each microvessel image represents a sparse subset of microbubble signals;
• and
• (e) generating a combined microvessel image based on a combination of the microvessel image for each of the plurality of subsets of microbubble signal data.