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US 12,538,880 B2Granted
Methods of in vitro culturing sphagnum
AssigneeMicropropagation Services (E.M.) Limited
InventorNeal Wright
Life of this patent
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Abstract
A method for in vitro culturing Sphagnum is provided. The method comprises providing a vegetative fragment of Sphagnum and surface cleaning the fragment with alcohol. The method further comprises dissecting the fragment to provide a piece (106) of Sphagnum. The method further comprises initiating the piece (106) of Sphagnum in a culture medium (104).
Claims19
A method for in vitro culturing Sphagnum, comprising:
- providing a vegetative Sphagnum;
- dissecting the vegatative Sphagnum to provide a vegetative fragment of Sphagnum having a length of at least 1 mm;
- after the dissecting the vegetative Sphagnum to provide the vegetative fragment, surface cleaning the vegetative fragment with alcohol;
- after the surface cleaning the vegetative fragment with alcohol, dissecting the vegetative fragment to provide a piece of Sphagnum having a length of less than 1 mm;
- and
- initiating the piece of Sphagnum in a culture medium.
3 more elements
Dependencies are inferred from the claim text.