Mass spectral tissue analysis
AssigneePurdue Research Foundation
InventorsRobert Graham Cooks, Livia Schiavinato Eberlin, Christina Ramires Ferreira, Allison Lisa Dill, Demian R. Ifa
Life of this patent
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Abstract
The invention generally relates to mass spectral analysis. In certain embodiments, methods of the invention involve analyzing a lipid containing sample using a mass spectrometry technique, in which the technique utilizes a liquid phase that does not destroy native tissue morphology during analysis.
Claims18
A method for assessing a tissue sample for presence of cancerous cells that are associated with brain, kidney, or prostate, the method comprising:
- producing mass spectral data using a desorption electrospray ionization (DESI) technique that comprises directing a liquid phase that does not destroy native tissue morphology from a DESI probe onto the tissue sample to thereby desorb one or more compounds from the sample that are analyzed by a mass spectrometer;
- receiving the mass spectral data of a tissue sample;
- producing a mass spectral image of the tissue sample based on the mass spectral data;
- receiving histochemical data of the tissue sample;
- producing an optical image of the tissue sample from the histochemical data;
- overlaying the mass spectral image of the tissue sample with the optical image of the tissue sample to produce an overlaid image;
- and
- assessing the overlaid image for presence of cancerous cells that are associated with at least one type of cancer selected from the group consisting of brain, kidney, and prostate.
A method for assessing a tissue sample for presence of cancerous cells that are associated with brain, kidney, or prostate, the method comprising:
- producing mass spectral data of a tissue sample comprising a cancerous section that is associated with brain, kidney, or prostate using a desorption electrospray ionization (DESI) technique that comprises directing a liquid phase that does not destroy native tissue morphology from a DESI probe onto the tissue sample to thereby desorb one or more compounds from the sample that are analyzed by a mass spectrometer;
- receiving the mass spectral data of the tissue sample comprising the cancerous section that is associated with brain, kidney, or prostate;
- producing a mass spectral image of the tissue sample comprising the cancerous section based on the mass spectral data;
- receiving histochemical data of the tissue sample comprising the cancerous section;
- producing an optical image of the tissue sample comprising the cancerous section from the histochemical data;
- overlaying the mass spectral image of the tissue sample comprising the cancerous section with the optical image of the tissue sample comprising the cancerous section to produce an overlaid image;
- identifying the cancerous section that is associated with brain, kidney, or prostate within the overlaid image;
- and
- assessing the overlaid image to determine a grade of the cancerous section that is associated with brain, kidney, or prostate.
Dependencies are inferred from the claim text.