Vaccine comprising protein-targeted (PT) extracellular DNA isolated from bacterial matrix

A vaccine comprising

• i) protein-targeted DNA (ptDNA), wherein said ptDNA is extracellular DNA isolated from the matrix of Pseudomonas aeruginosa, Escherichia coli, and/or Staphylococcus aureus matrix which promotes generation and/or enrichment of heat-resistant soluble proteins upon exposure to a bodily fluid, and wherein said ptDNA-comprising vaccine triggers the development of antibodies against said ptDNA, which antibodies prevent interaction of said ptDNA with extracellular proteins in bodily fluid(s), and/or
• ii) a heat-resistant soluble protein obtained by (a) contacting a bodily fluid sample with ptDNA, wherein said ptDNA is extracellular DNA isolated from the matrix of Pseudomonas aeruginosa, Escherichia coli, and/or Staphylococcus aureus, followed by (b) heating said bodily fluid sample for 5 seconds to 48 hours at a temperature from 43° C. to 200° C., followed by (c) isolating a soluble protein fraction.