Detection of fumarate-derived modification in a test sample
AssigneeUniversity of South Carolina
InventorNorma Frizzell Catledge
Life of this patent
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Abstract
Examination of a test sample to determine the presence or quantity of succination of proteins is described. Examination can be via protein hydrolysis in total succination determination or via enzymatic digestion of isolated proteins and determination of the presence or quantity of modified peptides. The methods can be utilized for determination of excessive succination of lymph system proteins, which can be utilized in prevention or early detection of lymphopenia. Methods can be utilized for test samples of subjects under treatment with dimethyl fumarate suffering from multiple sclerosis. Methods can be utilized as a determination that treatment of the subject with DMF should be slowed or stopped.
Claims17
A method for determining a succination level in a test sample, comprising:
- isolating one or more components from the test sample of a subject, wherein the isolated one or more components comprise peripheral blood mononuclear cells;
- hydrolyzing proteins of the test sample;
- examining the hydrolysis product according to a negative ion mode mass spectrometry analysis technique;
- and
- by use of the examination, determining the presence or quantity of free s-(2-succino) cysteine (2SC) in the test sample,
- wherein the subject has been treated with dimethyl fumarate or a monomethyl fumarate prodrug prior to determining the presence or quantity of free 2SC in the test sample.
Dependencies are inferred from the claim text.