Sample testing method and sample analyzer

A sample analyzer, comprising:

• a sampling portion configured to obtain a sample to be tested and transfer the sample to be tested to a reaction portion;
• a reagent supply portion configured to store a reagent and supply the reagent to the reaction portion as required, wherein the reagent comprises a hemolytic agent and comprises a first fluorescent dye and a third fluorescent dye for staining particles in the sample to be tested;
• the reaction portion comprising a mixing chamber and being configured to mix the sample to be tested and the reagent for reaction to form one sample solution to be tested;
• an optical detection system, comprising a first light source, a second light source, a flow cell, and an optical signal detector, wherein particles in the one sample solution to be tested are capable of flowing in the flow cell in a single test, the first light source is configured to irradiate the particles flowing in the flow cell by using light with a first wavelength and the second light source is configured to irradiate the particles flowing in the flow cell by using light with a second wavelength such that the particles generate optical signals, and the optical signal detector includes a scattered light signal detector, a first fluorescence detector and a third fluorescence detector, wherein the scattered light signal detector is configured to detect scattered light signals from the optical signals, the first fluorescence detector is configured to detect first fluorescence signals that are emitted by the particles in the one sample solution to be tested that have been stained with the first fluorescent dye under irradiation by the first light source, and the third fluorescence detector is configured to detect third fluorescence signals that are emitted by the particles in the one sample solution to be tested that have been stained with the third fluorescent dye under irradiation by the second light source;
• a transfer apparatus configured to transfer, to the flow cell of the optical detection system, the one sample solution to be tested that has been treated with the reagent in the reaction portion;
• and
• a processor configured to obtain a classification result and/or a counting result of white blood cells in the sample to be tested based on the first fluorescence signals and at least one type of the scattered light signals, wherein obtaining the classification result comprises classifying the white blood cells in the sample to be tested as lymphocytes, monocytes, neutrophils and eosinophils, and obtaining the counting result comprises after the white blood cells in the sample to be tested are classified as lymphocytes, monocytes, neutrophils and eosinophils, counting the lymphocytes, the monocytes, the neutrophils and the eosinophils separately, and to obtain a counting result of nucleated red blood cells in the sample to be tested based on the third fluorescence signals and at least one type of the scattered light signals.