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US 12,535,482 B2Granted
Method for labeling intracellular and extracellular targets of leukocytes
AssigneeBeckman Coulter, Inc.
InventorsFabrice Malergue, Andreas Van Agthoven
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Abstract
The present invention relates to methods for labeling intracellular and extracellular targets of leukocytes, as well as to kits for performing said methods.
Claims9
A kit for performing a method for labeling a marker of leukocytes, the kit comprising:
- (a) a first solution, comprising a compound, selected from the group consisting of formaldehyde, paraformaldehyde, and glutaraldehyde;
- (b) a second solution, comprising
- (i) a quaternary ammonium salt or an amine containing compound, selected from the group consisting of ammonium chloride (NH4Cl), glycine, tris (hydroxymethyl) aminomethane (Tris), and ethanolamine, or a mixture thereof and
- (ii) a first detergent,
- wherein the second solution has a pH of 6.0 to 6.6;
- and
- (c) a third solution, comprising
- (i) a compound, selected from the group consisting of formaldehyde, paraformaldehyde, and glutaraldehyde, and
- (ii) a second detergent, the second detergent comprising a C12 alkane residue, sodium N-lauroyl sarcosine, dextran sulfate, and a polvoxyethylene-polyoxypropylene block copolymer, or a mixtures thereof.
6 more elements
A kit for performing a method for labeling a marker of leukocytes, the kit comprising
- (a) a first solution comprising a cross-linking agent, wherein the cross-linking agent is formaldehyde at a concentration of 0.5% to 2% (v/v);
- (b) the second solution comprising
- (i) ammonium chloride (NH4Cl), and
- (ii) sodium N-lauroyl sarcosine in a concentration of 0.05% to 0.5% (w/v),
- wherein the second solution has a pH of 6.0 to 6.6;
- and
- (c) a third solution comprising
- (i) formaldehyde in a concentration of between 0.01% and 1% (v/v), and
- (ii) sodium N-lauroyl sarcosine in a concentration of 0.01% to 0.5% (w/v).
6 more elements
Dependencies are inferred from the claim text.