Absorbance spectroscopy analyzer and method of use

A spectroscopy analyzer, comprising:

• an illumination unit, comprising:
• a unit housing assembly that defines a unit internal space;
• a first light source within the unit internal space of the unit housing assembly, the first light source positioned on a first axis and configured to emit first light in a first light range of approximately 450 nm to approximately 680 nm;
• a second light source within the unit internal space of the unit housing assembly, positioned on a second axis that intersects and is angularly offset with respect to the first axis and configured to emit second light in at least a second light range of approximately 1050 nm to approximately 2500 nm;
• a third light source within the unit internal space of the unit housing assembly, the third light source positioned on the second axis, the third light source configured to emit third light in a third light range different than the second light range;
• a reflector within the unit internal space of the unit housing assembly, the reflector including an aperture through which the first axis extends such that the first light passes through the aperture and a reflective surface angled in relation to the first axis and second axis such that the second light and the third light reflect off the reflector;
• a focal lens within the unit internal space of the unit housing assembly positioned to receive and focus the first light passed through the aperture of the reflector, and to receive and focus the second light and the third light reflected from the reflective surface of the reflector;
• and
• an exit lens within the unit internal space of the unit housing assembly positioned to receive and focus the first light, the second light, and the third light from the focal lens into a specimen light;
• a sample holder having a microchannel configured to hold a sample of a fluid specimen and positioned such that the specimen light focused from the exit lens illuminates the sample, wherein the specimen light moving through the sample produces an analysis light;
• an optical element device positioned to receive at least a portion of the analysis light that passes through the sample, the optical element device comprising:
• a device housing assembly that defines a device internal space;
• a receiving lens within the device internal space of the device housing assembly positioned to receive and focus the analysis light;
• a dichroic mirror-reflector within the device internal space of the device housing assembly positioned to receive the focused analysis light from the receiving lens, the dichroic mirror-reflector configured to filter the analysis light such that a first portion of the analysis light in the first light range and the third light range is reflected off the dichroic mirror-reflector as a spectrometer light, and such that a second portion of the analysis light in the second light range passes through the dichroic mirror-reflector as a detector light;
• and
• an output lens within the device internal space of the device housing assembly positioned to receive and focus the spectrometer light;
• a spectrometer positioned to receive the focused spectrometer light from the output lens;
• and
• a detector positioned to receive the detector light through the dichroic mirror-reflector.