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US 12,534,755 B2Granted
Compositions and methods for performing hybridizations with separate denaturation of the sample and probe
AssigneeAgilent Technologies, Inc.
InventorSteen Hauge Matthiesen
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Abstract
The invention provides methods and compositions for separately denaturing a probe and target in hybridization applications. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization applications. Compositions for use in the invention include an aqueous composition comprising at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
Claims20
A method of hybridizing nucleic acid sequences comprising:
- combining a first nucleic acid sequence within a sample with a first aqueous composition comprising at least one polar aprotic solvent and at least one accelerating agent, wherein the polar aprotic solvent is in an amount effective to denature a double-stranded nucleotide sequence and is not dimethyl sulfoxide (DMSO);
- and
- combining said first nucleic acid sequence which has been combined with said first aqueous composition with a second nucleic acid sequence for at least a time period sufficient to hybridize the first and second nucleic acid sequences, wherein the polar aprotic solvent is selected from the group consisting of:
Dependencies are inferred from the claim text.