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US 12,534,735 B2Granted
Mannanase variants
AssigneeBASF SE
InventorsKatie Kline, Amanda Rae Logue, Asfia Qureshi, Cindy Hoang, Jesper Nielsen, Mark Miller, Tong Li
Life of this patent
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Abstract
A mannanase at least 75% identical to SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4, a polynucleotide encoding the mannanase, an expression construct comprising the polynucleotide, and a host cell comprising the polynucleotide or the expression construct.
Claims19
A mannanase variant comprising an amino acid sequence having at least 90% sequence identity to the full-length amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:
- 3, or SEQ ID NO:
- 4, wherein the mannanase variant comprises two or more amino acid substitutions selected from the group consisting of A31V, Q89V, N96D, A119Y/H/T, E264Q/V, W289F/M/H, N312F/Y, T348S/R/N/M/G, E349T/S/D/G, S352N/G, and D379V, wherein the numbering is according to SEQ ID NO:
- 2, and wherein the mannanase variant has mannan-degrading activity.
1 more element
Dependencies are inferred from the claim text.