Compositions and methods for enhanced protein production in bacillus licheniformis
AssigneeDanisco US Inc.
InventorsSteven D. Doig, Ryan L Frisch, Hongxian He, Chris Leeflang, Zhen Ma, Brian James Paul
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Abstract
The present disclosure is generally related to compositions and methods for constructing and/or obtaining B. licheniformis cells (e.g., protein production hosts) comprising enhanced protein production capabilities. Thus, certain embodiments are related to genetically modified Bacillus licheniformis strains derived from parental B. licheniformis strains producing increased amounts of one or more proteins of interest.
Claims14
A method for producing an increased amount of a protein of interest (POI) in a modified Bacillus licheniformis cell comprising:
- (a) modifying a parental B. licheniformis cell expressing a POI by introducing therein a polynucleotide comprising a native B. licheniformis prsA promoter operably linked to a native B. licheniformis prsA open reading frame (ORF), and
- (b) fermenting the modified cell under suitable conditions for the production of the POI,
- wherein the modified cell produces an increased amount of the POI relative to the parental cell when fermented under the same conditions, wherein the POI is a secreted protein.
A modified Bacillus licheniformis cell derived from a parental B. licheniformis cell comprising an endogenous B. licheniformis prsA gene encoding a native B. licheniformis prsA protein,
- wherein the modified cell further comprises an introduced polynucleotide comprising a native B. licheniformis prsA promoter operably linked to a native B. licheniformis prsA open reading frame (ORF),
- wherein the modified cell produces an increased amount of a secreted protein of interest relative to the parental cell when fermented under the same conditions.
Dependencies are inferred from the claim text.