Method for generating cardiac tissue mimetics

A method for generating a cardiac tissue mimetic, comprising the steps of

• a. providing cardiomyocytes (CM),
• b. providing fibroblasts (FB),
• c. mixing said cardiomyocytes (CM) and said fibroblasts (FB) at a ratio from 2.5:1 to 10:1, thereby providing a first mixture,
• d. incubating said first mixture as a hanging drop culture at 35° C. to 39° C. in a medium promoting growth of cardiomyocytes for 3 days to 6 days and/or incubating at 35° C. to 39° C. for 3 days to 6 days the hanging drop culture mixture in a well of a cell culture plate coated with a non-adhesive hydrogel, such that a spherical structure forms consisting of said cardiomyocytes and said fibroblasts,
• e. adding endothelial cells (EC) to said spherical structure at a ratio of cardiomyocytes (CM) to endothelial cells (EC) from 1.5:1 to 4:1, thereby providing a second mixture,
• f. incubating said second mixture in a well of a cell culture plate coated with a non-adhesive hydrogel at 35° C. to 39° C. in a 1:1 mixture of a medium promoting growth of cardiomyocytes and a medium promoting growth of vascular endothelial cells for 8 days to 30 days, such that the cardiac tissue mimetic is formed.