Method for maturation of retinal tissue containing continuous epithelium
AssigneesRiken, Racthera Co., Ltd.
InventorsHideya Sakaguchi, Yoshiki Sasai, Mototsugu Eiraku, Daiki Nukaya, Atsushi Kuwahara
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Abstract
The present invention provides a method for maintaining a continuous epithelial structure of a retinal tissue including culturing the retinal tissue in a medium comprising a methyl group donor or a substrate of the methyl group donor at a concentration at which cell differentiation of a neural retinal progenitor cell is suppressed, and a neurite extension inhibitor at a concentration at which neurite extension is suppressed.
Claims12
A method for maintaining a continuous epithelial structure of a human retinal tissue comprising culturing the human retinal tissue in a medium comprising (a) methionine at a concentration not less than 25 mg/L and not more than 75 mg/L, (b) corticosterone at a concentration of not less than 1 nM and not more than 1 μM, and (c) L-glutamic acid at a concentration of not more than 25 μM, wherein the human retinal tissue is derived from pluripotent stem cells, wherein the human retinal tissue is in a differentiation stage from an early developmental stage until a developmental stage when the proportion of NRL positive cells to the number of photoreceptor progenitor cells present in the human retinal tissue reaches 1-3%, and wherein the human retinal tissue does not have a rosette structure.
Dependencies are inferred from the claim text.