Modified Trichoderma fungal strain for the production of an enzyme cocktail

A modified Trichoderma fungus comprising:

• (1) deletion of an ACE1 gene encoding the polypeptide consisting of SEQ ID NO:2, and simultaneous insertion of an xyr1V821F gene at the ACE1 locus, resulting in disruption by deletion of the ACE1 gene and constitutive expression of transcription factor Xyr1 containing the V821F mutation;
• and
• (2) deletion of an SLP1 gene encoding the polypeptide consisting of SEQ ID NO:3, and simultaneous insertion of a Cel3a gene from Rasamsonia emersonii at the SLP1 locus, resulting in disruption by deletion of the SLP1 gene and expression of beta-glucosidase Cel3a as set forth in SEQ ID NO:4 4;
• wherein:
• SEQ ID NO:
• 2 is the sequence of the protein expressed by the ACE1 gene prior to disruption;
• SEQ ID NO:
• 3 is the sequence of the protein expressed by the SLP1 gene prior to disruption;
• and
• the modified strain of Trichoderma fungus has at least about 42 times greater activity of beta-glucosidase enzyme compared to unmodified Trichoderma fungus.