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US 12,534,499 B2Granted
Mesophilic argonaute systems and uses thereof
AssigneesThe Board of Trustees of the Leland Stanford Junior University, Intima Bioscience, Inc.
InventorsLei S. Qi, Modassir Choudhry, Xueqiu Lin, Trevor N. Collingwood, Thomas Henley, Benjamin Klapholz, Tilmann Buerckstuemmer, Sejla Salic
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Abstract
Constructs comprising Argonautes and neighboring genes are disclosed for use in gene editing. Disclosed are also compositions and methods utilizing these Argonautes and neighboring genes. Also disclosed are the methods of making and using the Argonautes and neighboring genes in treating various diseases, conditions, and cancer.
Claims9
A system comprising:
- a mesophilic Argonaute (Ago) polypeptide, or a polynucleic acid encoding the mesophilic Ago polypeptide, or a functional fragment or variant thereof, wherein the Ago polypeptide has an amino acid sequence set forth in any one of SEQ ID NOs 1-10;
- and
- an exogenous non-naturally occurring guiding polynucleic acid that binds a target polynucleic acid,
- wherein upon contacting the target polynucleic acid with the Ago polypeptide and the exogenous non-naturally occurring guiding polynucleic acid at a mesophilic temperature, the Ago polypeptide cleaves the target polynucleic acid.
1 more element
Dependencies are inferred from the claim text.