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US 12,534,494 B2Granted
Devices and methods for synthesis
AssigneeTwist Bioscience Corporation
InventorsAndres Fernandez, Eugene P. Marsh, Maryam Habibian
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Abstract
Provided herein are compositions, devices, systems and methods for electrochemical synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.
Claims20
A method for synthesizing a polynucleotide comprising:
- providing a solid support, the solid support including:
- a base layer comprising silicon,
- an intermediate layer comprising an oxide, and
- a top layer comprising platinum;
- wherein the intermediate layer is positioned between the base layer and the top layer, and wherein the top layer comprises a plurality of voids configured to allow fluid communication of a solvent with the intermediate layer;
- contacting at least one nucleoside attached to the intermediate layer of the solid support with a protected nucleoside, wherein the protected nucleoside is configured to form a covalent bond with the at least one nucleoside to generate a protected polynucleotide;
- applying a voltage to a solvent in fluid communication with the protected polynucleotide, wherein the voltage results in deprotection of a terminal nucleoside of the protected polynucleotide, and wherein the voltage is delivered as at least 2 pulses;
- and
- repeating the contacting step and the applying step to synthesize the polynucleotide.
6 more elements
A method for synthesizing a polynucleotide comprising:
- (a) providing a solid support, the solid support comprising:
- a base layer comprising silicon;
- an intermediate layer comprising an oxide;
- and
- a top layer, wherein the intermediate layer is positioned between the base layer and the top layer, and the top layer comprises a plurality of voids configured to allow fluid communication of a solvent with the intermediate layer;
- and
- wherein the intermediate layer comprises a first plurality of protected polynucleotides;
- (b) energizing at least one electrode in contact with the intermediate layer to electrochemically generate a deprotection reagent, wherein the deprotection reagent deprotects at least one polynucleotide of the first plurality of protected polynucleotides;
- (c) coupling at least one protected nucleoside to the at least one deprotected polynucleotide;
- and
- (d) repeating steps (a)-(c) to synthesize the polynucleotide.
8 more elements
Dependencies are inferred from the claim text.