US 12,534,447 B2 · Bioconjugates and antibodies for immunodetection assisted by derivatization of the mycotoxin patulin

Abstract

The present invention relates to bioconjugates and labeled derivatives of the adducts formed by the reaction of patulin with benzene-1,2-dithiols or their salts. Said conjugates are suitable for the production of antibodies capable of recognizing the aforementioned patulin-(benzene-1,2-dithiols) adducts with high affinity and specificity. Likewise, the present invention also relates to the use of bioconjugates of patulin-(benzene-1,2-dithiols) adducts and of labeled derivatives thereof as assay antigens. Furthermore, the present invention also relates to immunochemical methods for the analysis of patulin, using the bioconjugates and labeled derivatives and antibodies of the invention, which include a previous step to quantitatively convert patulin into these adducts. This invention also provides a kit for analyzing patulin comprising a benzene-1,2-dithiol or its salts, antibodies against patulin-(benzene-1,2-dithiols) adducts, and assay antigens.

Claims37

Dependent claims

A bioconjugate of general formula (I):

[T-L-Z]m—P (I)
where:
T is selected from the group consisting of the radicals R-I and R-II wherein R1, R2, R3, and R4 are independently selected from H, substituted or unsubstituted aryl groups, linear or branched alkyl groups of 1 to 6 carbon atoms, OMe, OEt, OPr, C≡N, CO2H, CO2-alkyl of 1 to 6 carbon atoms, NO2, NH2, F, Cl, Br, and I
L is a hydrocarbon chain, saturated or unsaturated, of 0 to 14 carbon atoms, where the chain is linear or branched, and said hydrocarbon chain comprises the substitution of between 0 and 7 carbon atoms by heteroatoms, which are selected from the group consisting of S, O and N,
Z is a functional group selected from:
—NH—, —NH(C═O)—, —NH(C═O)O—, —(C═O)NH—, —(C═O)O—, —O(C═O)—, —O(C═O)O—, —O(C═O)NH—, —S—,
P is a natural or synthetic peptide or polypeptide, or a protein, with a molecular weight greater than 2000 daltons,
m is a number with a value between 1 and 500.

5 more elements

Dependencies are inferred from the claim text.

Bioconjugates and antibodies for immunodetection assisted by derivatization of the mycotoxin patulin

Life of this patent

Bioconjugates and antibodies for immunodetection assisted by derivatization of the mycotoxin patulin

Life of this patent

Abstract

Claims37