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US 12,533,425 B2Granted
Methods and systems using peptides for targeting and mapping human nerves in image guided surgery, diagnostics and therapeutic delivery
AssigneeAlume Biosciences, Inc.
InventorBrett J. Berman
Life of this patent
Estimating the term…
Abstract
The present invention provides methods for guiding preservation of human neurons or human nerves during surgery, as well as labeling and mapping nerves in targeted organs for map-guided surgery, by administering a fluorescently-labeled peptide that specifically binds to the human neurons or human nerves. The invention further provides human neuron or nerve targeting molecules comprising fluorescently-labeled peptides that specifically bind to human neurons or human nerves and compositions thereof.
Claims24
A method of labeling and locating a pulmonary nerve surrounding a bronchus in a human subject, comprising:
- (a) contacting the pulmonary nerve surrounding the bronchus with a human neuron or nerve targeting molecule, wherein the human neuron or nerve targeting molecule comprises:
- a peptide selected from the group consisting of SGQVPWEEPYYVVKKSS (HNP 401;
- SEQ ID NO:
- 1), WEYHYVDLNWTSQHPQ (HNP 402;
- SEQ ID NO:2), DLPDIIWDFNWETA (HNP 403;
- SEQ ID NO:3), DTHAHAKPRVPAFKSV (HNP 404;
- SEQ ID NO:16), Ac-SGQVPWEEPYYVVKKSSGGC (HNP401 with GGC linker;
- SEQ ID NO:4), Ac-WEYHYVDLNWTSQHPQGGC (HNP402 with GGC linker;
- SEQ ID NO:5), Ac-DLPDIIWDFNWETAGGC (HNP403;
- with GGC linker SEQ ID NO:6), Ac-QVPWEEPYYVVKKSSGGC (HNP401-N-2 with GGC linker;
- SEQ ID NO:7), Ac-PWEEPYYVVKKSSGGC (HNP401-N-4 with GGC linker;
- SEQ ID NO:8), Ac-EEPYYVVKKSSGGC (HNP401-N-6 with GGC linker;
- SEQ ID NO:9), Ac-PYYVVKKSSGGC (HNP401-N-8 with GGC linker;
- SEQ ID NO:10), Ac-SGQVPWEEPYYVVKKGGC (HNP401-C-2 with GGC linker;
- SEQ ID NO:11), Ac-SGQVPWEEPYYVVGGC (HNP401-C-4 with GGC linker;
- SEQ ID NO:12), Ac-SGQVPWEEPYYGGC (HNP401-C-6 with GGC linker;
- SEQ ID NO:
- 13), Ac-SGQVPWEEPGGC (HNP401-C-8 with GGC linker;
- SEQ ID NO:14), QVPWEEPYYVVKKSS (HNP401-N-2;
- SEQ ID NO:20);
- QVPWEEPYYVVKKSSGG (HNP401-N-2 with GG linker;
- SEQ ID NO:21), PWEEPYYVVKKSS (HNP401-N-4;
- SEQ ID NO:
- 22);
- EEPYYVVKKSS (HNP401-N-6;
- SEQ ID NO:23), PYYVVKKSS (HNP401-N-8;
- SEQ ID NO:
- 24), SGQVPWEEPYYVVKK (HNP401-C-2;
- SEQ ID NO:25), SGQVPWEEPYYVV (HNP401-C-4;
- SEQ ID NO:26), SGQVPWEEPYY (HNP401-C-6;
- SEQ ID NO:27), SGQVPWEEP (HNP401-C-8;
- SEQ ID NO:28), PWEEPYYVVKKSSGG (HNP401-N-4 with GG linker;
- SEQ ID NO:118), EEPYYVVKKSSGG (HNP401-N-6 with GG linker;
- SEQ ID NO:
- 119), PYYVVKKSSGG (HNP401-N-8 with GG linker;
- SEQ ID NO:120), SGQVPWEEPYYVVKKGG (HNP401-C-2;
- with GG linker;
- SEQ ID NO:121), SGQVPWEEPYYVVGG (HNP401-C-4 with GG linker;
- SEQ ID NO:122), SGQVPWEEPYYGG, (HNP401-C-6 with GG linker;
- SEQ ID NO:123), and SGQVPWEEPGG (HNP401-C-8 with GG linker;
- SEQ ID NO:124);
- and a cargo comprising a fluorescent moiety, thereby labeling the pulmonary nerve surrounding the bronchus;
- (b) illuminating the cargo through a wall of the bronchus within a lung;
- receiving fluoresced light from the cargo;
- and using the received fluoresced light to determine a location of the pulmonary nerve surrounding the bronchus;
- and
- (c) using the location of the pulmonary nerve surrounding the bronchus to guide irradiating or ablating of the pulmonary nerve surrounding the bronchus.
44 more elements
A method of labeling and locating a renal nerve within a renal artery wall in a kidney of a human subject, comprising:
- (a) contacting the renal nerve within the renal artery wall with a human neuron or nerve targeting molecule, wherein the human neuron or nerve targeting molecule comprises:
- a peptide selected from the group consisting of SGQVPWEEPYYVVKKSS (HNP 401;
- SEQ ID NO:1), WEYHYVDLNWTSQHPQ (HNP 402;
- SEQ ID NO:2), DLPDIIWDFNWETA (HNP 403;
- SEQ ID NO:
- 3), DTHAHAKPRVPAFKSV (HNP 404;
- SEQ ID NO:
- 16), Ac-SGQVPWEEPYYVVKKSSGGC (HNP401 with GGC linker;
- SEQ ID NO:4), Ac-WEYHYVDLNWTSQHPQGGC (HNP402 with GGC linker;
- SEQ ID NO:5), Ac-DLPDIIWDFNWETAGGC (HNP403;
- with GGC linker SEQ ID NO:6), Ac-QVPWEEPYYVVKKSSGGC (HNP401-N-2 with GGC linker;
- SEQ ID NO:7), Ac-PWEEPYYVVKKSSGGC (HNP401-N-4 with GGC linker;
- SEQ ID NO:8), Ac-EEPYYVVKKSSGGC (HNP401-N-6 with GGC linker;
- SEQ ID NO:9), Ac-PYYVVKKSSGGC (HNP401-N-8 with GGC linker;
- SEQ ID NO:10), Ac-SGQVPWEEPYYVVKKGGC (HNP401-C-2 with GGC linker;
- SEQ ID NO:11), Ac-SGQVPWEEPYYVVGGC (HNP401-C-4 with GGC linker;
- SEQ ID NO:12), Ac-SGQVPWEEPYYGGC (HNP401-C-6 with GGC linker;
- SEQ ID NO:
- 13), Ac-SGQVPWEEPGGC (HNP401-C-8 with GGC linker;
- SEQ ID NO:14), QVPWEEPYYVVKKSS (HNP401-N-2;
- SEQ ID NO:20);
- QVPWEEPYYVVKKSSGG (HNP401-N-2 with GG linker;
- SEQ ID NO:21), PWEEPYYVVKKSS (HNP401-N-4;
- SEQ ID NO:
- 22);
- EEPYYVVKKSS (HNP401-N-6;
- SEQ ID NO:23), PYYVVKKSS (HNP401-N-8;
- SEQ ID NO:
- 24), SGQVPWEEPYYVVKK (HNP401-C-2;
- SEQ ID NO:25), SGQVPWEEPYYVV (HNP401-C-4;
- SEQ ID NO:26), SGQVPWEEPYY (HNP401-C-6;
- SEQ ID NO:27), SGQVPWEEP (HNP401-C-8;
- SEQ ID NO:28), PWEEPYYVVKKSSGG (HNP401-N-4 with GG linker;
- SEQ ID NO:118), EEPYYVVKKSSGG (HNP401-N-6 with GG linker;
- SEQ ID NO:
- 119), PYYVVKKSSGG (HNP401-N-8 with GG linker;
- SEQ ID NO:120), SGQVPWEEPYYVVKKGG (HNP401-C-2;
- with GG linker;
- SEQ ID NO:121), SGQVPWEEPYYVVGG (HNP401-C-4 with GG linker;
- SEQ ID NO:122), SGQVPWEEPYYGG, (HNP401-C-6 with GG linker;
- SEQ ID NO:123), and SGQVPWEEPGG (HNP401-C-8 with GG linker;
- SEQ ID NO:124);
- and a cargo comprising a fluorescent moiety, thereby labeling the renal nerve within the renal artery wall;
- (b) illuminating the cargo through the renal artery wall;
- receiving fluoresced light from the cargo;
- and using the received fluoresced light to determine a location of the renal nerve within the renal artery wall;
- and
- (c) using the location of the renal nerve within the renal artery wall to guide irradiating or ablating of the renal nerve within the renal artery wall.
45 more elements
Dependencies are inferred from the claim text.