Process, tube and device for the preparation of wound healant composition

A method of treating a patient, the method comprising:

• providing a system for the preparation of a cell composition of platelet rich plasma and hyaluronic acid, the system comprising:
• a tube having:
• an anticoagulant adapted to reduce coagulation of whole blood;
• and
• a hyaluronic acid, wherein prior to introduction of the whole blood into the tube, the anticoagulant and the hyaluronic acid are at least partially filled in the tube with the hyaluronic acid beneath the anticoagulant, and wherein during centrifugation the hyaluronic acid migrates above the platelet rich plasma;
• the tube sterilized with the hyaluronic acid and hermetically sealed;
• the tube adapted to receive the whole blood while maintaining sterility;
• and
• the tube adapted to be centrifuged for a length of time in order to separate platelet rich plasma from the whole blood, and to prepare the cell composition including the platelet rich plasma and the hyaluronic acid in the tube;
• withdrawing the whole blood from the patient;
• centrifuging the whole blood in the tube to form the cell composition including the platelet rich plasma and the hyaluronic acid;
• and
• administering the cell composition to the patient;
• wherein the platelet rich plasma is autologous.