Method for producing cell aggregate including glial progenitor cells

A method for producing a cell aggregate including glial progenitor cells, comprising:

• (1) a step of subjecting pluripotent stem cells to suspension culture in an embryoid-body-forming culture medium containing SMAD signaling inhibitors and a Wnt signaling activator in the absence of feeder cells for 7 days, to form a cell aggregate, wherein the SMAD signaling inhibitors are a TGFβ inhibitor and a BMP inhibitor, wherein the TGFβ inhibitor is SB431542, wherein the BMP inhibitor is LDN-193189, and wherein the Wnt signaling activators is CHIR99021;
• (2) a step of subjecting the cell aggregate obtained in (1) to suspension culture in an embryoid-body-forming culture medium containing retinoic acid for 7 days;
• (3) a step of subjecting the cell aggregate obtained in (2) to suspension culture in an embryoid-body-forming culture medium or neuron-and-glia-proliferating culture medium containing retinoic acid and a SHH signaling activator, wherein the SHH signaling activator is purmorphamine;
• and
• (4) a step of subjecting the cell aggregate obtained in (3) to suspension culture in a neuron-and-glia-proliferating culture medium containing no retinoic acid and purmorphamine.