Kit for detecting soluble growth stimulation expressed gene 2 protein

A detection kit for soluble growth stimulation expressed gene 2 (sST2) protein, comprising:

• a first reagent,
• a second reagent,
• wherein, the first reagent comprises:
• 1% w/v Dodecyl alcohol polyoxyethylene ether,
• 0.1% w/v sodium azide,
• 5 KU/L lipase,
• 2% w/v sodium cholate,
• 5 mmol/l CaCl2,
• 2.0% w/v methacryloyloxy phosphatidylcholine,
• 3% w/v blocker Heterophilic Blocking Reagent, and
• 200 mmol/l Tris (hydroxymethyl) aminomethane buffer, pH 7.4;
• the second reagent comprises:
• 0.15% w/v latex microspheres,
• 20 μg/ml anti-sST2 antibody Fab fragment,
• 100 mmol/L glycine buffer pH 8.0,
• 0.1% w/v NaCl,
• 0.5% w/v methacryloyloxy phosphatidylcholine and
• 0.1% w/v NaN3;
• wherein,
• the latex microspheres comprise surface functional groups selected from the group consisting of:
• carboxyl, sulfhydryl, chloromethyl, and any combination thereof;
• the average particle size of the latex microspheres is 350 nm to 450 nm;
• the anti-sST2 antibody is selected from the group consisting of:
• non-affinity IgG antibody, affinity IgG antibody and IgY antibody;
• the anti-sST2 antibody is derived from murine, rabbit, goat, camel or avian;
• and
• the anti-sST2 antibody or antigen-binding fragment thereof is covalently bound to the surface of the latex microspheres.