Fast pulsing for nanopore sensors
AssigneeIllumina, Inc.
InventorBoyan Boyanov
Life of this patent
Estimating the term…
Abstract
Sequencing systems and methods are provided that include a nanopore sensor that includes a cis well associated with a cis electrode and a trans well associated with a trans electrode, a membrane separating the cis well and the trans well, and a nanopore well embedded in the membrane providing a channel through the membrane; a command node connected directly to the nanopore sensor. The command node is configured to apply a potential across the nanopore well and a command pulse. The system further includes an amplifier with a feedback loop coupled to the nanopore sensor and a switch disposed between the amplifier and the nanopore sensor. The switch is driven by a clock pulse and configured to ground an inverting input of the amplifier.
Claims34
A sequencing system, comprising:
- a nanopore sensor comprising a cis well associated with a cis electrode and a trans well associated with a trans electrode, a membrane separating the cis well and the trans well, and a nanopore well embedded in the membrane providing a channel through the membrane;
- a node connected directly to the nanopore sensor, wherein the node is configured to apply a potential across the nanopore well and a first voltage pulse;
- an amplifier coupled to the nanopore sensor;
- and
- a switch disposed between the amplifier and the nanopore sensor, wherein the switch is configured to be driven by a clock pulse and to ground an inverting input of the amplifier.
A sequencing system, comprising:
- a nanopore sensor comprising a cis well associated with a cis electrode and a trans well associated with a trans electrode, a membrane separating the cis well and the trans well, and a nanopore well embedded in the membrane providing a channel through the membrane;
- a node connected directly to the nanopore sensor, wherein the node is configured to apply a potential across the nanopore well and a first voltage pulse;
- an amplifier with a feedback loop coupled to the nanopore sensor;
- a switch disposed across the feedback loop, wherein the switch is configured to be driven by a clock pulse and to bypass an inverting loop when the switch is closed.
Dependencies are inferred from the claim text.