Imaging system hardware
Assignee10x Genomics, Inc.
InventorsZachary Bent, David Maurice Cox
Life of this patent
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Abstract
A sample holder includes a first member featuring a first retaining mechanism configured to retain a first substrate that includes a sample, a second member featuring a second retaining mechanism configured to retain a second substrate that includes a reagent medium, and an alignment mechanism connected to at least one of the first and second members, and configured to align the first and second members such that the sample contacts at least a portion of the reagent medium when the first and second members are aligned.
Claims19
A method of processing a tissue sample supported on a sample region of a substrate, the substrate secured between a first member and a second member of a substrate holder, wherein the first member comprises:
- a base configured to receive the substrate;
- a first lateral wall extending from the base;
- a second lateral wall extending from the base and opposite the first lateral wall, wherein the first lateral wall and the second lateral wall each have a first longitudinal length;
- a third lateral wall extending from the base;
- a fourth lateral wall extending from the base and opposite the third lateral wall, wherein the third lateral wall and the fourth lateral wall each have a second longitudinal length, wherein the first longitudinal length is greater than the second longitudinal length;
- and
- at least one tab extending laterally outward from and along at least a portion of the first lateral wall;
- and wherein
- the second member comprises:
- a main body comprising a first longitudinal side and a second longitudinal side, the main body extending from the first longitudinal side to the second longitudinal side;
- a first aperture formed in the main body;
- and
- at least one notch extending laterally outward from and along at least a portion of the first longitudinal side;
- wherein the method comprises:
- positioning the substrate on the first member;
- releasably locking the second member to the first member by releasably locking each tab of the at least one tab to a corresponding notch of the at least one notch thereby forming a first well around at least a portion of the sample region;
- and
- after the releasably locking the second member to the first member, contacting the tissue sample with a reagent by introducing the reagent into the first well through the first aperture.
Dependencies are inferred from the claim text.