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US 12,529,089 B2Granted
Compositions and methods for disease diagnosis using single cell analysis
AssigneePattern Bioscience, Inc.
InventorsNicolas Arab, Ross Johnson
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Abstract
Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.
Claims12
A method for identifying a cell comprising:
- (a) contacting a sample containing cells with a first reporter that is a pH indicating fluorophore, thereby forming a sample mixture;
- (b) partitioning the sample mixture into a plurality of volume partitions containing one cell or one cellular aggregate;
- (c) incubating the volume partitions for a period of time at a specified temperature or series of temperatures;
- (d) monitoring optical characteristics of the volume partitions during the incubation time;
- (e) constructing waveforms for the volume partitions based on the optical characteristics over time;
- and
- (f) identifying the cell in the volume partition based on the waveform constructed for the volume partition.
4 more elements
Dependencies are inferred from the claim text.