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AssigneeAmyris, Inc.
InventorsGale A. Wichmann, Sean Lund, Joshua Lerman, Hanxiao Jiang, Yi Xiong
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Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.
A genetically modified host cell capable of producing one or more steviol glycosides comprising a heterologous nucleic acid encoding an ABC-transporter having transporter activity and comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:
A genetically modified host cell capable of producing one or more steviol glycosides, comprising:
Dependencies are inferred from the claim text.