O-phosphoserine export protein variant and method for producing O-phosphoserine, cysteine, and derivatives thereof using the same
AssigneeCj Cheiljedang Corporation
InventorsHye Min Park, So-Yeon Kim, Hee-jin Sim, Jin Nam Lee
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Abstract
An O-phosphoserine (OPS) export protein variant with increased O-phosphoserine exporting activity, and a method for producing O-phosphoserine, cysteine, and cysteine derivatives using the same. Also provided herein are O-phosphoserine-producing microorganisms, a polynucleotide encoding the O-phosphoserine export protein variant, and a vector containing the polynucleotide encoding the O-phosphoserine export protein variant.
Claims12
A polypeptide having O-phosphoserine (OPS) exporting activity, comprising an amino acid sequence having at least 95% or higher sequence identity to the amino acid sequence of SEQ ID NO:
- 11, wherein
- a) the polypeptide comprises a substitution of isoleucine (I) at a position corresponding to 241 in the amino acid sequence of SEQ ID NO:
- 11 with threonine (T), a substitution of aspartic acid (D) at a position corresponding to 246 in the amino acid sequence of SEQ ID NO:
- 11 with valine (V), and a substitution of valine (V) at a position corresponding to 330 in the amino acid sequence of SEQ ID NO:
- 11 with isoleucine (I),
- b) the amino acid at a position corresponding to 88 in the amino acid sequence of SEQ ID NO:
- 11 is phenylalanine, and
- c) the amino acid at a position corresponding to 207 in the amino acid sequence of SEQ ID NO:
- 11 is lysine (K).
Dependencies are inferred from the claim text.