Microorganisms and methods for improving product yields on methanol using acetyl-CoA synthesis

A non-naturally occurring microbial organism having a methanol metabolic pathway, an acetyl-CoA pathway, and a formate assimilation pathway wherein said methanol metabolic pathway comprises a methanol dehydrogenase or a methanol methyltransferase, wherein said methanol dehydrogenase catalyzes conversion of methanol to formaldehyde and wherein said methanol methyltransferase catalyzes conversion of methanol into methyl-THF,

• wherein said acetyl-CoA pathway comprises a xylulose-5-phosphate phosphoketolase wherein said xylulose-5-phosphate phosphoketolase catalyzes conversion of xylulose-5-phosphate (Xu5P) and phosphate to acetyl-phosphate (ACTP) and glyceraldehyde-3-phosphate (G3P),
• wherein said formate assimilation pathway comprises:
• (1) a pyruvate formate lyase, wherein said pyruvate formate lyase catalyzes conversion of pyruvate to acetyl-CoA (ACCOA);
• or
• (2) a pyruvate dehydrogenase, pyruvate ferredoxin oxidoreductase, or pyruvate:
• NADP+ oxidoreductase, and a formate dehydrogenase, wherein said pyruvate dehydrogenase, pyruvate ferredoxin oxidoreductase, or pyruvate:
• NADP+ oxidoreductase catalyzes conversion of pyruvate to acetyl-CoA (ACCOA), and where said formate dehydrogenase converts CO2 to formate;
• wherein an enzyme of the methanol metabolic pathway, an enzyme of the acetyl-CoA pathway, and at least one enzyme of the formate assimilation pathway is encoded by an exogenous nucleic acid and is expressed in a sufficient amount to increase the yield of acetyl-CoA per mole of methanol if compared to the yield of acetyl-CoA per mole of methanol in the absence of said enzymes.