Viral vectors for nuclear reprogramming

A Paramyxoviridae viral vector for reprogramming somatic cells into iPSCs, wherein said vector comprises negative-strand RNA comprising a first sequence that produces a mRNA molecule encoding a first reprogramming factor, a second sequence that produces a mRNA molecule encoding a second reprogramming factor, a third sequence that produces a mRNA molecule encoding a Paramyxoviridae virus N polypeptide, a fourth sequence that produces a mRNA molecule encoding a Paramyxoviridae virus P polypeptide, a fifth sequence that produces a mRNA molecule encoding a Paramyxoviridae virus M polypeptide, a sixth sequence that produces a mRNA molecule encoding a Paramyxoviridae virus F polypeptide, and a seventh sequence that produces a mRNA molecule encoding a Paramyxoviridae virus L polypeptide, wherein one or more of said mRNA molecules comprises a nucleic acid sequence complementary to a microRNA (miRNA) associated with pluripotency located within a 3′ untranslated region of said one or more mRNA molecules, wherein said miRNA is miR375, wherein said first and second sequences are located between said sixth and seventh sequences, wherein said miRNA is endogenous to said iPSCs and in an amount sufficient to (a) reduce a level of said vector within said iPSCs or (b) reduce expression of one or more of said first and said second reprogramming factors in said iPSCs.