Selection marker-comprising cell line and uses thereof for protein production
AssigneeSanofi
InventorsBruno Louis Dumas, Mohammed Nabil Lounis
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Abstract
The present invention concerns a cell line comprising an endogenous dehydroorotate dehydrogenase (DHODH) gene which is partially or fully inactivated, and its use for producing recombinant proteins.
Claims23
A cell line comprising an endogenous dihydroorotate dehydrogenase (DHODH) gene which is partially or fully inactivated, wherein the cell line is selected from Chinese Hamster Ovary (CHO) cell lines, HEK293, HKB11, PER-C6, HT1080, Daudi, Raji, and CAP cell lines, wherein the cell line further comprises an expression vector comprising a nucleotide sequence encoding an exogenous mammalian DHODH and at least one expression cassette for expressing a recombinant protein.
Dependencies are inferred from the claim text.