Method for producing induced pluripotent stem cells

A method of generating iPS cells having a rearranged γδ-TCR gene, comprising the following steps 1) to 3):

• i) stimulating collected blood cells in a medium with any one kind or a plurality of kinds of interleukins selected from IL-2, IL-15, and IL-23, and a bisphosphonate;
• ii) introducing into the blood cells a Sendai virus (SeV) vector comprising at least four genes encoding cell reprogramming factors;
• and
• iii) culturing the cells having introduced therein the genes and thereby generating the iPS cells having the rearranged γδ-TCR gene, wherein
• the bisphosphonate comprises any one kind or a plurality of kinds selected from zoledronic acid, pamidronic acid, alendronic acid, risedronic acid, ibandronic acid, incadronic acid, and minodronic acid,
• the method is free of a step of purifying or enriching γδ-TCR gene-positive cells by a cell sorter, and
• the method is free of a step of treating the collected blood cells with an antibody against γδ-TCR before the steps i) to iii),
• the proportion of γδ T cells contained in the cell population obtained after step i) is 3.73% or less, and
• the iPS cells having a rearranged γδ-TCR gene are generated in at least 3 out of 7 cell lines established after step iii).