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US 12,528,944 B2Granted
Live-cell fluorescent mitochondrial stains
AssigneeHoward Hughes Medical Institute
InventorsLuke D. Lavis, Sambashiva Banala, Ariana N. Tkachuk, Timothy A. Brown
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Abstract
Fluorescent stains are described, which enable imaging of cellular structures without the need for genetic manipulation. Unique diaminobenzopyrylium dyes are disclosed, together with their use as live-cell mitochondrial stains.
Claims3
A compound of the formula:
- wherein
- L is selected from the group consisting of alkyl, substituted alkyl, and —(CH2CH2O)nCH2CH2—, wherein n is from 1 to 4;
- Z and Z′ are selected from the group consisting of O(alkyl) and N(alkyl)2;
- R1 and R1′ are selected from the group consisting of halogen, H, D, alkyl, and substituted alkyl;
- or R1 and Z, and R1′ and Z′, taken together with the carbon atoms to which they are bonded, form a substituted or unsubstituted ring containing 5, 6, 7, 8, or 9 atoms;
- R2 and R2′ are selected from the group consisting of halogen, H, D, alkyl, and substituted alkyl;
- or R2 and Z, and R2′ and Z′, taken together with the carbon atoms to which they are bonded, form a substituted or unsubstituted ring containing 5, 6, 7, 8, or 9 atoms;
- R3 and R3′ are selected from the group consisting of alkyl and substituted alkyl;
- or R3, taken together with the nitrogen atoms to which it is bonded, and R3′, taken together with the nitrogen atoms to which it is bonded, form substituted or unsubstituted rings containing 4, 5, 6, 7, 8, or 9 atoms, wherein L is disposed between the ring of R3 and the ring of R3′;
- R4 and R4′ are selected from the group consisting of H, halogen, CH3, CF3, CN, COO(alkyl), COO(aryl), alkyl, substituted alkyl, aryl, substituted aryl, alkenyl, and substituted alkenyl;
- and R5 and R5′ are selected from the group consisting of H, halogen, CH3, CF3, CN, COO(alkyl), COO(aryl), alkyl, substituted alkyl, aryl, substituted aryl, alkenyl, and substituted alkenyl;
- or R4 and R5, and R4′ and R5′, taken together with the carbon atoms to which they are bonded, form substituted or unsubstituted rings containing 5, 6, 7, 8, or 9 carbon atoms.
9 more elements
Dependencies are inferred from the claim text.