Biotin-containing monomers and articles formed therefrom
AssigneeThermo Fisher Bioprocessing Inc.
InventorJerald K. Rasmussen
Life of this patent
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Abstract
Biotin-containing monomers, polymeric materials formed from the biotin-containing monomers, articles containing the polymeric materials, methods of making the articles, and methods of using the articles are provided. The articles can be used, for example, for affinity capture of biotin-binding proteins, including biotin-binding fusion proteins (i.e., a biotin-binding protein fused to another biomaterial). Articles that contain captured biotin-binding proteins can be further used for affinity capture of various biotin-containing biomaterials such as biotinylated proteins. The articles can also be used, for example, for affinity capture of biotin-binding fusion proteins where the fusion protein includes, for example, an enzyme or antibody.
Claims17
A polymeric material comprising monomeric units derived from a monomer of Formula (I)
- in an amount ranging from 1 to 100 mole percent based on total moles of monomeric units in the polymeric material,
- wherein
- Q is oxygen or NH;
- X is —(CO)—O— or —(CO)—NH—
- Y is —NH—(CO)—, —NH—(CO)—NH—, —O—(CO)—NH—, or —O—(CO)—;
- Z is —O— or —NH—;
- R1 is hydrogen or methyl;
- and
- R2 is an alkylene, alkylene substituted with an aryl, or heteroalkylene, with the proviso that if the polymeric material is a homopolymer and Y is equal to —NH—(CO)—, then R2 has greater than 2 carbon atoms;
- and
- each R3 and R4 is each independently a (hetero) alkylene.
An article comprising:
- a substrate;
- and
- a polymeric material disposed on the substrate, the polymeric material comprising monomeric units derived from a monomer of Formula (I)
- in an amount ranging from 1 to 100 mole percent based on total moles of monomeric units in the polymeric material,
- wherein
- Q is oxygen or NH;
- X is —(CO)—O— or —(CO)—NH—
- Y is —NH—(CO)—, —NH—(CO)—NH—, —O—(CO)—NH—, or —O—(CO)—;
- Z is —O— or —NH—;
- R1 is hydrogen or methyl;
- R2 is an alkylene, alkylene substituted with an aryl, or heteroalkylene;
- and
- each R3 and R4 is each independently a (hetero) alkylene.
A monomer of Formula (I)
- wherein
- Q is oxygen or NH;
- X is —(CO)—O— or —(CO)—NH—
- Y is —NH—(CO)—, —NH—(CO)—NH—, —O—(CO)—NH—, or —O—(CO)—;
- Z is —O—;
- R1 is hydrogen or methyl;
- R2 is an alkylene, alkylene substituted with an aryl, or heteroalkylene with the proviso that if Y is —NH—(CO)—, then R2 has greater than 2 carbon atoms;
- and
- each R3 and R4 is each independently a (hetero) alkylene.
Dependencies are inferred from the claim text.