Gel patterned surfaces
AssigneeIllumina, Inc.
InventorsSteven M. Barnard, M. Shane Bowen, Maria Candelaria Rogert Bacigalupo, Wayne N. George, Andrew A. Brown, James Tsay
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Abstract
Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.
Claims21
An array, comprising:
- a substrate;
- a patterned sol-gel layer disposed on the substrate;
- wherein the patterned sol-gel layer comprises an array of a plurality of wells;
- and
- a gel material disposed in each of the plurality of wells, wherein the gel material is adapted to promote capture and/or amplification of target nucleic acids.
A patterned surface, comprising:
- a substrate;
- a patterned layer disposed on the substrate comprising an array of a plurality of wells and interstitial regions separating each of the plurality of wells;
- and
- a gel material coating the wells;
- wherein the array of the plurality of wells comprises analyte features and fiducial features;
- and wherein the interstitial regions are devoid of the gel material.
A patterned surface, comprising:
- a substrate;
- and
- a patterned layer disposed on the substrate, wherein:
- the patterned layer comprises a planar surface interrupted by one or more concave features,
- the one or more concave features are bordered by one or more interstitial regions on the planar surface;
- and
- the one or more concave features are coated with a polymer, with the interstitial regions being devoid of the polymer;
- wherein the patterned layer comprises a plurality of analyte features and fiducial features,
- and wherein primer nucleic acids are attached to the polymer.
Dependencies are inferred from the claim text.