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US 12,527,894 B2Granted
Thin film interposition of basement membrane scaffolds
AssigneeIVIVA Medical, Inc.
InventorCharles C. Klassen
Life of this patent
Estimating the term…
Abstract
Disclosed are compositions and methods of making basement membrane constructs having interior or luminal volumes. The interior or luminal volumes may be in the form of vascular networks for liquid (e.g., blood) or gas perfusion. The interior spaces may also contain cells, such as epithelial cells. Also disclosed are tissues and organs, and methods of making thereof, comprising basement membrane constructs.
Claims22
A composition comprising a thin film having a thickness of about 100 μm or less, wherein:
- the thin film comprises functional basement membrane material comprising (i) collagen, (ii) gelatin, (iii) hydrogel, (iv) polylactic acid, (v) chitosan, (vi) nitrocellulose, or (vii) liquified or homogenized decellularized tissue, organ or extracellular matrix material (ECM);
- the thin film defines a boundary between a first interior volume and a second interior volume, wherein the first interior volume or second interior volume forms a vascular channel network;
- and
- the thin film is capable of fluid filtration, gas diffusion, secretion or absorption of an electrolyte, and/or diffusion of a hormone across the thin film.
1 more element
A method of making a composition, comprising:
- a. generating a first sacrificial structure comprising one or more sacrificial materials on a support structure or a first thin film layer having a thickness of about 100 μm or less;
- b. applying a second thin film layer having a thickness of about 100 μm or less comprising functional basement membrane material to the first sacrificial structure, wherein the first sacrificial structure contacts a first side of the second thin film layer, wherein the functional basement membrane material comprises (i) collagen, (ii) gelatin, (iii) hydrogel, (iv) polylactic acid, (v) chitosan, (vi) nitrocellulose, or (vii) liquified or homogenized decellularized tissue, organ or extracellular matrix material (ECM);
- c. applying a second sacrificial structure comprising one or more sacrificial materials to a second side of the second thin film layer to form a second sacrificial structure;
- d. embedding the product of steps (a), (b) and (c) in a sacrificial or permanent material or applying a third thin film to the second sacrificial material;
- and
- e. removing the first and second sacrificial structures to provide a first interior volume and a second interior volume having a boundary defined by the second thin film layer, wherein at least one of the first interior volume and second interior volume comprises a vascular channel network, thereby making the composition.
3 more elements
Dependencies are inferred from the claim text.