Generation of kidney glomerular specific endothelial cells and methods of use
AssigneeCornell University
InventorsShahin Rafii, David M. Barry, Sina Rabbany, Balvir Kunar, Raphael Lis
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Abstract
The present disclosure is directed to method of generating human glomeruli endothelial cells (HGECs) from human endothelial cells (ECs), comprising expressing in human ECs an exogenous nucleic acid encoding a T-box transcription factor 3 (Tbx3), alone or in combination with one or more of PR domain zinc finger protein 1 (Prdm1), GATA Binding Protein 5 (Gata5) and Pre-B-Cell Leukemia Transcription Factor 1 (Pbx1). Disclosed also are HGECs produced by the methods of the instant disclosure, as well as methods for using the same.
Claims24
A method of generating human glomeruli endothelial cells (HGECs) from human endothelial cells (ECs), comprising
- expressing in human ECs an exogenous nucleic acid encoding a T-box transcription factor 3 (Tbx3) polypeptide, or a polypeptide having at least 85% amino acid sequence identity to the Tbx3 polypeptide, and
- obtaining HGECs that display the phenotypic marker profile:
- Ehd3+Mapt+Sox17+Mest+Igfbp5+Kdr+VE-cadherin+ (Ehd3:
- EH Domain Containing 3, Mapt:
- Microtubule Associated Protein Tau, Sox17:
- SRY-Box Transcription Factor 17, Mest:
- Mesoderm Specific Transcript, Igfbp5:
- Insulin Like Growth Factor Binding Protein 5, Kdr:
- Kinase Insert Domain Receptor, and VE-cadherin:
- Vascular Endothelial cadherin).
Dependencies are inferred from the claim text.