Promoting regeneration and transformation in plants
AssigneesKWS SAAT SE & Co. KGaA, Stichting Wageningen Research
InventorsJixiang Kong, Susana Martin-Ortigosa, David Pacheco Villalobos, Christine Kastner, Frank Ludewig, Anneke Horstman, Kimberly Anne Boutilier
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus and shoot formation and regeneration of plants using sodium nitroprusside (SNP) in combination with Brassica napus BABYBOOM (BnBBM).
Claims14
A method of promoting somatic embryogenesis or organogenesis of a plant, comprising the following steps:
- (a) inducing callus formation from at least one plant cell comprising incubating the at least one plant cell in the presence of sodium nitroprusside (SNP), and
- (b) introducing into the at least one plant cell to be used in step (a) or into at least one cell of the callus obtained in step (a) an expression cassette comprising a coding nucleotide sequence selected from
- (i) the nucleotide sequence of SEQ ID NO:
- 1;
- and
- (ii) a nucleotide sequence encoding a polypeptide having the amino acid sequence of SEQ ID NO:
- 2,
- wherein the nucleotide sequence is operably linked to a heterologous constitutive regulatory element or a heterologous inducible regulatory element;
- and
- (c) cultivating the callus obtained in step (b) under conditions promoting growth of embryos and/or shoots out of the callus, wherein in the callus the polypeptide is expressed from the expression cassette constitutively or upon induction of the heterologous inducible regulatory element.
A method of using a SNP in combination with a coding nucleotide sequence selected from the group consisting of:
- (i) the nucleotide sequence of SEQ ID NO:
- 1;
- and
- (ii) a nucleotide sequence encoding a polypeptide having the amino acid sequence of SEQ ID NO:
- 2,
- wherein the nucleotide sequence is operably linked to a heterologous constitutive regulatory element or a heterologous inducible regulatory element,
- (a) in a method for inducing callus formation from at least one plant cell from Beta vulgaris, Zea mays or Spinacia oleracea, or in a method for somatic embryogenesis or indirect regeneration of a Beta vulgaris plant, Zea mays plant or Spinacia oleracea plant, from callus;
- (b) in a method of transformation of a plant cell from Beta vulgaris, Zea mays or Spinacia oleracea, or in a method of modifying the genome of a plant cell from Beta vulgaris, Zea mays or Spinacia oleracea;
- or
- (c) in the production of a transgenic plant cell, plant or seed from Beta vulgaris, Zea mays or Spinacia oleracea, or in the production of a genetically modified plant cell, plant or seed from Beta vulgaris, Zea mays or Spinacia oleracea.
Dependencies are inferred from the claim text.