Imaging system and imaging range adjustment method

An imaging range adjustment method for imaging a change in color by a reaction of digital PCR for a plurality of measurement targets arranged on a plane, the imaging range adjustment method being executed by an imaging system, the imaging system having:

• a light source that irradiates the plurality of measurement targets with light;
• a photodetector that detects light from the plurality of measurement targets;
• one or more lenses;
• an adjustment mechanism that focuses imaging on the plurality of measurement targets;
• a drive mechanism that changes relative position of the photodetector and the plurality of measurement targets;
• and
• an analysis mechanism that performs digital count measurement, the plurality of measurement targets having a same shape and a same size, the plurality of measurement targets being arranged at equal pitches in a longitudinal direction and a lateral direction on the plane, the imaging range adjustment method comprising:
• performing focusing on the plurality of measurement targets by the drive mechanism in a depth direction;
• acquiring an image by imaging the plurality of measurement targets by means of the photodetector;
• rotating at least one of the plurality of measurement targets or the photodetector by the drive mechanism such that the plurality of measurement targets are arranged in a horizontal direction or a vertical direction within the image;
• changing the relative position of the photodetector and the plurality of measurement targets in either a horizontal axial direction or a vertical axial direction orthogonal to the horizontal axial direction and the depth direction by the drive mechanism, based on a peak shape of at least one of a peak of maximum intensity or a peak of minimum intensity among peaks in a histogram of pixel intensities in the image;
• and
• changing the relative position to the other of the horizontal axial direction or the vertical axial direction by the drive mechanism, based on a peak shape of at least one of the peak of maximum intensity or the peak of minimum intensity among the peaks in the histogram of pixel intensities in the image.

An imaging range adjustment method for imaging a change in color by a reaction of digital PCR for a plurality of measurement targets arranged on a plane, the imaging range adjustment method being executed by an imaging system, the imaging system having:

• a light source that irradiates the plurality of measurement targets with light;
• a photodetector that detects light from the plurality of measurement targets;
• one or more lenses;
• an adjustment mechanism that focuses imaging on the plurality of measurement targets in a depth direction;
• a drive mechanism that changes relative position of the photodetector and the plurality of measurement targets;
• and
• an analysis mechanism that performs digital count measurement,
• the plurality of measurement targets having a same shape and a same size, the plurality of measurement targets being arranged at equal pitches in a longitudinal direction and a lateral direction on the plane,
• the imaging range adjustment method comprising:
• performing focusing on the plurality of measurement targets by the drive mechanism;
• acquiring an image by imaging the plurality of measurement targets by means of the photodetector;
• rotating at least one of the plurality of measurement targets or the photodetector by the drive mechanism such that the plurality of measurement targets are arranged in a horizontal direction or a vertical direction within the image;
• changing the relative position in either a horizontal axial direction or a vertical axial direction orthogonal to the horizontal axial direction and the depth direction by the drive mechanism based on a standard deviation of pixel intensities in the image;
• and
• changing the relative position to the other of the horizontal axial direction or the vertical axial direction orthogonal to the first axial direction by the drive mechanism based on a standard deviation of pixel intensities in the image.